常用生物缓冲液的缓冲范围表
Buffer |
MW |
pKa 20℃ |
Working range |
delta pKa per 10℃ |
MES |
195.2 |
6.15 |
5.5-6.7 |
-0.110 |
Bis-Tris |
209.2 |
6.5 |
5.8-7.2 |
|
ADA |
190.2 |
6.60 |
6.0-7.2 |
-0.110 |
PIPES |
302.4 |
6.80 |
6.1-7.5 |
-0.085 |
ACES |
182.2 |
6.90 |
6.1-7.5 |
-0.200 |
MOPSO |
225.3 |
6.9 |
6.2-7.6 |
|
Imidazol-HCl |
68.08 |
6.95 |
6.2-7.8 |
|
Bis-Tris Propane |
282.3 |
6.8* |
6.3-9.5 |
|
BES |
213.2 |
7.15 |
6.4-7.8 |
-0.160 |
MOPS |
209.3 |
7.20 |
6.5-7.9 |
-0.013 |
TES |
229.2 |
7.50 |
6.8-8.2 |
-0.200 |
HEPES |
238.3 |
7.55 |
6.8-8.2 |
-0.140 |
DIPSO |
243.3 |
7.6 |
7.0-8.2 |
|
TAPSO |
259.3 |
7.6 |
7.0-8.2 |
|
HEPPSO |
268.3 |
7.8 |
7.1-8.5 |
|
POPSO |
362.4 |
7.8 |
7.2-8.5 |
|
TEA |
149.2 |
7.8 |
7.3-8.3 |
|
EPPS |
252.3 |
8.0 |
7.3-8.7 |
|
Tricine |
179.2 |
8.15 |
7.4-8.8 |
-0.210 |
Tris |
121.1 |
8.30 |
7.0-9.1 |
-0.310 |
Bicine |
163.2 |
8.35 |
7.6-9.0 |
-0.180 |
TAPS |
243.3 |
8.4 |
7.7-9.1 |
|
Glycylglycine |
|
8.40 |
|
-0.280 |
AMPSO |
227.3 |
9.0 |
8.3-9.7 |
|
CHES |
207.3 |
9.3 |
8.6-10.0 |
|
CAPSO |
237.3 |
9.6 |
8.9-10.3 |
|
AMP |
89.1 |
9.7 |
9.0-10.5 |
|
CAPS |
221.3 |
10.4 |
9.7-11.1 |
|